Growth Conditions for Alpha-amylase-producing Bacterium PLS 75 Strain Isolated from a High Temperature and Saline Area

Teuku M. Iqbalsyah, Jasmin P. Nabila, Febriani

2019

Abstract

Extreme habitat has been explored to find microorganisms that are capable of producing industrial enzymes with better activity and stability. Four thermo-halophilic bacteria strains isolated from undersea fumaroles were screened for their ability to produce extracellular hydrolytic enzymes. The most potent α-amylase producer was PLS 75 strain. The strain was Gram-negative with uniform basil-chained shaped. Homology analysis of the 16S rRNA gene fragments shows that the strain was closely related to an uncultured bacterium isolate, supporting the fact that the strain was difficult to culture. Fermentation study shows that PLS 75 produced the highest biomass (5.48 mg/ml) and α-amylase activity (4.43 U/ml) when incubated at 60 °C, pH 7 for 31 h. The results provided information on PLS 75 culture conditions that may be used to produce the enzyme for the study of its attributes and catalytic activity.

Paper Citation

in Harvard Style

Iqbalsyah T., Nabila J. and Febriani. (2019). Growth Conditions for Alpha-amylase-producing Bacterium PLS 75 Strain Isolated from a High Temperature and Saline Area. In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 368-375. DOI: 10.5220/0010182900002775

in Bibtex Style

@conference{imc-scimath19,
author={Teuku M. Iqbalsyah and Jasmin P. Nabila and Febriani},
title={Growth Conditions for Alpha-amylase-producing Bacterium PLS 75 Strain Isolated from a High Temperature and Saline Area},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={368-375},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010182900002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - Growth Conditions for Alpha-amylase-producing Bacterium PLS 75 Strain Isolated from a High Temperature and Saline Area
SN - 978-989-758-556-2
AU - Iqbalsyah T.
AU - Nabila J.
AU - Febriani.
PY - 2019
SP - 368
EP - 375
DO - 10.5220/0010182900002775