Mechanisms and Electron Transfer of Prokaryotic Nitrate Reductases

Yiming Luo

2022

Abstract

There are three types of prokaryotic nitrate reductases which are essential in the biological nitrogen cycle, respectively membrane-bound respiratory nitrate reductase (Nar), periplasmic nitrate reductase (Nap) and prokaryotic assimilatory nitrate reductase (Nas). This work summarizes the structures of these three types of nitrate reductases, as well as their nitrate reduction mechanism and electron transfer pathways. They each contain a molybdenum bis-molybdopterin guanine dinucleotide cofactor (Mo-bis-MGD) as the active site for nitrate binding, and they use different numbers and types of iron–sulfur clusters to aid the electron transfer. Nar and Nap use hemes and the quinol–quinone Q cycle as the electron source, whereas Nas relies on flavodoxin or NADH. This work also presents some possible aspects for future research, such as the uniqueness of molybdenum, the large potential barriers in the Nap electron transfer pathway, and unsolved crystal structures of some enzyme subunits that have limited understanding.

Paper Citation

in Harvard Style

Luo Y. (2022). Mechanisms and Electron Transfer of Prokaryotic Nitrate Reductases. In Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB, ISBN 978-989-758-595-1, pages 505-513. DOI: 10.5220/0011219400003443

in Bibtex Style

@conference{icbeb22,
author={Yiming Luo},
title={Mechanisms and Electron Transfer of Prokaryotic Nitrate Reductases},
booktitle={Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,},
year={2022},
pages={505-513},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011219400003443},
isbn={978-989-758-595-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,
TI - Mechanisms and Electron Transfer of Prokaryotic Nitrate Reductases
SN - 978-989-758-595-1
AU - Luo Y.
PY - 2022
SP - 505
EP - 513
DO - 10.5220/0011219400003443