N-acetylcysteine (NAC) Inhibits ROS-Induced Ferroptosis in CTNS Knockdown β-Cells in Vitro

Hongrui Liang, Jingyu Xu, Yanxu Chen, Jingjie Yu, Ruoxun Peng

2022

Abstract

CTNS silence or mutation in the human body leads to cystinosis, an autosomal recessive lysosomal storage disease. Ferroptosis is an iron-dependent form of programmed cell death. N-acetylcysteine (NAC), a common antioxidant, is the acetylated precursor of L-cysteine. There are no studies about the relationship between cystinosis and ferroptosis yet. Therefore, in this paper, we aimed to 1) find out the relationship between CTNS knockdown and ferroptosis in ß-cells and 2) verify that NAC is a potential agent to protect CTNS knockdown ß-cells and is also a potent ferroptosis inhibitor. Since we do not have the access to do the experiments in a laboratory, all the following results and conclusions are hypothetical or from existing papers: CTNS-targeting siRNA inhibit cystinosin expression at mRNA and protein level; CTNS knockdown induces ferroptosis in ßcells and NAC attenuates ferroptosis; NAC attenuates oxidative stress in CTNS knockdown ß-cells; NAC restores energy level and glucose-stimulated insulin secretion in CTNS knockdown ß-cells. We conclude that, in vitro, the NAC pretreatment can effectively rescue CTNS knockdown ß-cells from ferroptosis by elevating the GPX4 mRNA and its protein level.

Paper Citation

in Harvard Style

Liang H., Xu J., Chen Y., Yu J. and Peng R. (2022). N-acetylcysteine (NAC) Inhibits ROS-Induced Ferroptosis in CTNS Knockdown β-Cells in Vitro. In Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB, ISBN 978-989-758-595-1, pages 1235-1243. DOI: 10.5220/0011390800003443

in Bibtex Style

@conference{icbeb22,
author={Hongrui Liang and Jingyu Xu and Yanxu Chen and Jingjie Yu and Ruoxun Peng},
title={N-acetylcysteine (NAC) Inhibits ROS-Induced Ferroptosis in CTNS Knockdown β-Cells in Vitro},
booktitle={Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,},
year={2022},
pages={1235-1243},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011390800003443},
isbn={978-989-758-595-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,
TI - N-acetylcysteine (NAC) Inhibits ROS-Induced Ferroptosis in CTNS Knockdown β-Cells in Vitro
SN - 978-989-758-595-1
AU - Liang H.
AU - Xu J.
AU - Chen Y.
AU - Yu J.
AU - Peng R.
PY - 2022
SP - 1235
EP - 1243
DO - 10.5220/0011390800003443